Call us now : +91 80 28528900, 01, 02


DeQuantoTM Ranibizumab (Lucentis ) PK ELISA KIT (Catalog # PK1006)

CATALOG # : PK1006

Pack Size : 1 X 96 Wells

QTY : 1

PRICE : Enquire

  • Product Specifications

    The PK (PharmacoKinetics) ELISA Kit is specifically designed to detect the amount/ concentration of the drug Ranibizumab in human serum/plasma

    • Sensitivity: 9.7 ng/ml
    • Very cost effective
    • Assay Duration:3.15-3.30 Hrs
    • Assay range: 1250 - 9.77 ng/ml
    • Assay Matrix: Tested and validated for Human serum or plasma
    • Specificity: Specifically to Ranibizumab drug
    • Note: "Lucentis®" is a registered trademark of Genentech.

    The kit is based on very robust ELISA technique and with very high sensitivity.

  • Product Summary


    Ranibizumab (Lucentis) is a recombinant human IgG1 monoclonal antibody fragment (Fab) that blocks angiogenesis by inhibiting vascular endothelial growth factor-A (VEGF-A) isoforms. The humanized anti-VEGF monoclonal antibody. Lucentis® is a registered trademark of Genentech. Ranibizumab, has been approved by the FDA for treatment of patients with wet age-related macular degeneration.

    Currently, the most commonly used VEGF antagonists are ranibizumab (Lucentis, Genentech, Inc., South San Francisco, CA) and bevacizumab (Avastin; Genentech, Inc., South San Francisco, CA). Ranibizumab, which is an antibody fragment form the bevacizumab molecule with an increased binding affinity for all forms of VEGF, has been approved for the treatment of patients with neo-vascular AMD by the Food and Drug Administration and by the European Medicines Agency since 2006 and 2007, respectively

    EMA Bio-analytical Method Validation Guidelines and industry-recommended practices for ligand-binding assays were used for validation of this kit. This Ranibizumab ELISA kit has been developed for specific quantification of Ranibizumab concentration in human serum or plasma with high sensitivity and reproducibility

    Assay Principle

    The DeQuantoTM Ranibizumab (Lucentis) PK ELISA kit is an ELISA based immunoassay. Standards and diluted samples (serum/ plasma) are incubated in the polystyrene microtiter plate pre coated with recombinant Human vascular endothelial growth factor-A (rhVEGF-A). The Ranibizumab present in the samples binds to the coated rhVEGF-A. Non bound or the excess substances are removed by washing. Horseradish Peroxidase (HRP) labeled antibody is added to the wells which binds to the rhVEGF-A-Ranibizumab complex in the wells. Following a wash to remove any unbound antibody-enzyme reagent, substrate solution is added to the wells. A color product is formed in proportion to the amount of Ranibizumab present in the sample or standards. The color development is stopped by addition of a stop solution. The absorbance is measured at 450 nm in a microtiter plate reader. The concentration of Ranibizumab in a sample can be interpolated from the standard curve.